| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Bacillus coahuilensis | AATGGGGATGGCAGCTGTC | ACCTCTCCGTGTTTAGGTGC | 60.08 | 59.68 | 193 | 125 |
100.00%
|
100.00%
|
| Primer 2 | Bacillus coahuilensis | AATGGGGATGGCAGCTGT | ACCTCTCCGTGTTTAGGTGC | 58.90 | 59.68 | 193 | 104 |
100.00%
|
100.00%
|
| Primer 3 | Bacillus coahuilensis | AATGGGGATGGCAGCTGTC | CCTCTCCGTGTTTAGGTGCT | 60.08 | 59.39 | 192 | 61 |
100.00%
|
100.00%
|
| Primer 4 | Bacillus coahuilensis | AGCTGTCGTTGCAAGGGA | TCGAATGAGTTCCTTGGCGA | 59.17 | 59.39 | 159 | 53 |
100.00%
|
100.00%
|
| Primer 5 | Bacillus coahuilensis | AATGGGGATGGCAGCTGTC | TCGAATGAGTTCCTTGGCGA | 60.08 | 59.39 | 171 | 53 |
100.00%
|
100.00%
|
| Primer 6 | Bacillus coahuilensis | AATGGGGATGGCAGCTGT | TCGAATGAGTTCCTTGGCGA | 58.90 | 59.39 | 171 | 42 |
100.00%
|
100.00%
|